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June 5, 2017

Dr. Thomas H. Johengen, ACT Chief Scientist

University of Michigan CILER

4840 South State Rd

Ann Arbor, MI 48108

Re: Nutrient Challenge Company Response Letter

Dear Dr. Johengen,

Real Tech Inc. would like to thank you and the Alliance for Coastal Technologies (ACT) team for the

opportunity to participate in the Nutrient Challenge competition and comment on the performance of the

Real Nitrate Analyzer GL series.

We are very pleased that ACT has organized this challenge and shined a spotlight on the benefits of real-

time sensors for environmental nutrient monitoring. Real Tech’s analyzers utilize spectrophotometry to

detect nitrate nitrogen in water without the use of reagents, are highly modifiable based on clients’ needs,

and can generate data at a frequency of every minute. Below we expand on some of the most important

aspects of Real Tech’s experiences in this challenge.

1) Design Application

Real Tech’s nitrate monitoring systems are designed for and most commonly used at well water blending

stations, and municipal and industrial water/wastewater treatment facilities. Due to the current regulations

and the typical nitrate concentrations encountered in these environments, two of the field tests

(Chesapeake Bay, MD and Kaneohe Bay, HI) were not within the design specifications of our analyzer.

Both of these field test sites had high salinity and very low nitrate concentrations (less than 0.04 mgN/L

compared to 10 mgN/L maximum allowable concentration in drinking water).

2) Custom Calibration

Real Tech’s preferred method of deployment for its analyzers involves an initial in-house calibration

followed by an on-site audit of the calibration after installation. This allows for an adjustment of the

calibration for background water interferences. However, as deployment prior to the actual testing days was

not a possibility and collecting audit data during the testing period would interfere with the testing, we were

not able to implement a correction based on on-site data. We strongly believe that spectrophotometric

measurement techniques work best when a custom calibration is built on-site. For instance, in the Maumee

River field test, Real-NO3 measurements appear to have tracked the actual nitrate concentrations well,

however, it is evident that a slight offset was present. This offset could have been corrected for with an on-

site correction factor on the first day of installation, had on-site data auditing been allowed. Furthermore,

continued collaboration between the client and the manufacturer will provide superior data as our analyzers

are capable of building a site-specific library which improves performance over time.

3) Range

Real Tech’s analyzers come with adjustable flow cell components. This allows for a customized approach to

concentration range. A longer path length flow cell increases sensitivity and accuracy at low concentrations

while a shorter path length flow cell provides the widest range, but has reduced sensitivity at low

concentrations. During the laboratory testing part of the nutrient challenge, we were asked to provide an

analyzer that would measure a range of 0.01-50 mgN/L NO

3

. We provide analyzers that can measure up to

hundreds of mgN/L. However, as explained above, a wider range comes with a trade-off of losing the

required sensitivity at low concentrations. For this reason, Real Tech participated in the laboratory testing

with an 8-mm flow cell that aimed to maintain a reasonable level of accuracy at low concentrations (0.01-0.1