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Ref. No. [UMCES] CBL 2016-010

ACT VS16-01

8

Lab-based Stability Test

A laboratory stability test was conducted to examine potential instrument drift in a non-biofouling

environment. These results are contrasted to the stability of measurement accuracy observed in the

long-term field mooring deployments. The test occurred over 56 days, with daily temperature

fluctuations of approximately 10

o

C, achieved by alternating the set point of the recirculation chiller.

Reference samples were collected at minimum and maximum temperatures at least 3 times per week.

The test was conducted in deionized water at saturated air conditions. Tanks were well circulated and

open to the atmosphere. Water in the test tank was exchanged as needed if there was any indication of

biological growth. Instruments stayed continuously submerged and were not exposed to air during any

water exchange. The goal of

comparisons of accuracy over time between the field and a sensor

deployed similarly in the laboratory is intended to provide insight into drift and reliability intrinsic to

the instrument relative to changes that may result from biofouling.

Moored Field Tests

Field Deployment Sites and Conditions

A four month moored deployment was conducted at Michigan Technological University’s

Great Lakes Research Center dock in Houghton, MI. Instruments were deployed in January and kept

under ice cover until April. Instruments were programmed to sample at a minimum frequency of once

per hour. ACT collected reference samples twice per day for 4 days per week during the entire

deployment. Instruments were moored at approximately 4m depth and surface access through the ice

was maintained by gentle circulation with a propeller to allow deployment of the Van Dorn sampling

bottle. The goal of this test application was to demonstrate instrument performance (reliability,

accuracy, and stability) in winter-time environmental conditions and to demonstrate the ability to

operate continuous observations under ice.

A three month moored deployment was conducted at the Chesapeake Biological Lab Pier,

Solomons, MD. Instruments were deployed between May and August during a period of warming

temperatures and high biological production. Instruments were moored at fixed depth of 1m on a

floating dock. Instruments were programmed to sample at a minimum frequency of once per hour.

ACT collected reference samples twice per day for 3 days per week and collected six samples on one

day per week during the entire deployment. The intensive sampling was spaced to capture the

maximum range of expected diurnal variation in dissolved oxygen concentrations. The goal of this test

application was to demonstrate instrument performance (reliability, accuracy, and stability) under high

biofouling conditions and over a range of salinity and temperature conditions in a coastal estuarine

environment.

A four month moored deployment was conducted in a shore patch reef at the Hawaii Institute

of Marine Biology (HIMB), Coconut Island, Kaneohe, HI. Instruments were deployed between

September and January. Instruments were moored at approximately 1m depth on a bottom mounted

PVC rack and were programmed to sample at a minimum frequency of once per hour. Some

manufacturers chose to sample more frequently to demonstrate that capability. ACT collected

reference samples twice per day for 3 days per week and collected six samples on one day per week

during the entire deployment. The intensive sampling was spaced to capture the maximum range of

expected diurnal variation in dissolved oxygen concentrations. The goal of this test application was to