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Ref. No. [UMCES] CBL 2016-013

ACT VS16-04

5

with corresponding DO concentrations of 8.6 and 12.6 mg/L, respectively. Two profiling trials

were conducted at each location. The first trial involved equilibrating test instruments at the

surface (3m) for ten minutes and then collecting three Niskin bottle samples at one minute

intervals. Following the third sample, the rosette was quickly profiled into the hypolimnion where

samples were collected immediately upon arrival and then each minute for the next 6 minutes. The

second trial was performed in the reverse direction. For Muskegon Lake, the range in

measurement differences between instrument and reference was -0.15 to 1.78 mg/L for cast 2 and -

0.92 to -0.17 mg/L for cast 3 (cast 1 was aborted and redone as cast 3. For Lake Michigan, the

range in measurement differences was -3.03 to 0.40 mg/L for cast 1 and -0.81 to 3.05 mg/L for cast

2. The equilibration rate of the sensor differed across each of the profiles with no obvious trend

across temperature or DO levels.

BACKGROUND AND OBJECTIVES

Instrument performance verification is necessary so that effective existing technologies can

be recognized and so that promising new technologies can be made available to support coastal

science, resource management and ocean observing systems. To this end, the NOAA-funded

Alliance for Coastal Technologies (ACT) serves as an unbiased, third party testbed for evaluating

sensors and sensor platforms for use in coastal environments. ACT also serves as a comprehensive

data and information clearinghouse on coastal technologies and a forum for capacity building

through workshops on specific technology topics (visit

www.act-us.info

).

As part of our service to the coastal community, ACT conducted a performance verification

of commercially available, in situ dissolved oxygen (DO) sensors through the evaluation of

objective and quality assured data. The goal of ACT’s evaluation program is to provide technology

users with an independent and credible assessment of instrument performance in a variety of

environments and applications. To this end, the data and information on performance

characteristics were focused on the types of information users most need.

The fundamental objectives of this Performance Verification were to: (1) highlight the

potential capabilities of particular in situ DO sensors by demonstrating their utility in a range of

coastal environments; (2) verify the claims of manufacturers on the performance characteristics of

commercially available DO sensors when tested in a controlled laboratory setting, and (3) verify

performance characteristics of commercially available DO sensors when applied in real world

applications in a diverse range of coastal environments.

INSTRUMENT TECHNOLOGY TESTED

Onset’s HOBO U26 Dissolved Oxygen Logger uses the RDO® Basic Technology

developed by In-Situ, Inc. This technology represents the latest advancements in optical Dissolved

Oxygen (DO) measurement. By improving upon the breakthrough optical DO sensor technology

that In-Situ brought to the environmental market in early 2004, the RDO Basic sensor provides

higher quality data and more robust performance than traditional methods. The “Basic” version of

the RDO used in the HOBO U26 provides the RDO advantages in an affordably-priced stand-alone

data logger.

Optical sensor technology uses LED lights, a lumiphore and a detector. A source of light

excites a lumiphore which luminesces, emitting a light of a different wavelength. Typically,