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Ref. No. [UMCES] CBL 2016-012

ACT VS16-03

5

users with an independent and credible assessment of instrument performance in a variety of

environments and applications. To this end, the data and information on performance

characteristics were focused on the types of information users most need.

The fundamental objectives of this Performance Verification were to: (1) highlight the

potential capabilities of particular in situ DO sensors by demonstrating their utility in a range of

coastal environments; (2) verify the claims of manufacturers on the performance characteristics of

commercially available DO sensors when tested in a controlled laboratory setting, and (3) verify

performance characteristics of commercially available DO sensors when applied in real world

applications in a diverse range of coastal environments.

INSTRUMENT TECHNOLOGY TESTED

The HydroCAT CTD with optical dissolved oxygen (ODO) Sensor is part of the model 37

family of moored sensors from Sea-Bird Scientific. It was released by Sea-Bird in 1997 and to date

over 10,000 instruments have been deployed. In 2012 Sea-Bird released the SBS63 which is an

optical dissolved oxygen sensor. The HydroCAT is designed for long duration deployment at

fixed-sites.

The optical dissolved oxygen sensor on the HydroCAT is individually calibrated in a

temperature-controlled bath. Bath temperatures are varied at each of 4 oxygen values, providing a

comprehensive 24-point calibration. Two reference sensors in each bath are standardized against

Winkler titrations. Response time tests are conducted on each sensor using gas. Salinity and

pressure impacts on sensor response are each checked at two separate points. By locating the ODO

sensor in the flow path the optimum correlation with the CTD measurements can be achieved. The

elapsed time between the CTD and associated oxygen measurement is easily quantified, and

corrected for in post-processing. The plumbing’s black tubing blocks light and further reduces in-

situ algal growth. Due to the stable sensor technology and comprehensive factory calibration the

HydroCAT does not require user calibration. Factory calibration is recommended every 1 – 2

years.

With its on-board data storage capability, internal battery pack and anti-foulant cartridges,

the HydroCAT can operate autonomously over long-term deployments. Data can be output in real-

time via SDI-12 or RS232.

The supplied

Universal Coastal Interface (UCI)

software provides easy set-up and

configuration, graphical real-time data display for pre-deployment checks and interactive sampling.

UCI can also be used with other sensors such as the SUNA V2 and HydroCAT-EP.

PERFORMANCE EVALUTION TEST PLAN

Laboratory Tests

Laboratory tests of accuracy, precision, response time, and stability were conducted at

Moss Landing Marine Lab. All tests were run under ambient pressure (logged hourly from a

barometer at the laboratory) and involved the comparison of dissolved oxygen concentration

reported by the instrument versus Winkler titration values of water samples taken from the test

baths. All tests were run in thermally controlled tanks at specific temperature, salinity, and DO