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Ref. No. [UMCES] CBL 2016-011

ACT VS16-02

5

14 mg/L. A linear regression of the composited data (r

2

= 0.998; p<0.0001)) had a slope of 0.987

and intercept of -0.150.

The PME miniDOT was evaluated in a profiling field test in the Great Lakes at two

separate locations in order to experience transitions from surface waters into both normoxic and

hypoxic hypolimnion. In Muskegon Lake, the temperature ranged from 21.0

o

C at the surface to

13.5

o

C in the hypolimnion, with corresponding DO concentrations of 7.8 and 2.8 mg/L,

respectively. In Lake Michigan, the temperature ranged from 21.0

o

C at the surface to 4.1

o

C in the

hypolimnion, with corresponding DO concentrations of 8.6 and 12.6 mg/L, respectively. Two

profiling trials were conducted at each location. The first trial involved equilibrating test

instruments at the surface (3m) for ten minutes and then collecting three Niskin bottle samples at

one minute intervals. Following the third sample, the rosette was quickly profiled into the

hypolimnion where samples were collected immediately upon arrival and then each minute for the

next 6 minutes. The second trial was performed in the reverse direction. For Muskegon Lake, the

miniDOT exhibited a negative bias in the colder, low DO hypolimnion and a positive bias in the

warm, normoxic surface water over both of the trials. The miniDOT appeared to reach

equilibration after 7 minutes but still exhibited final offsets of approximately 0.2 mg/L following

the profiled transitions. The range in measurement differences between instrument and reference

was -0.24 to 0.75 mg/L for cast 2 and -0.57 to 0.14 mg/L for cast 3 (cast 1 was aborted and redone

as cast 3). For Lake Michigan, during cast 1 the miniDOT was well matched during surface

equilibration and then exhibited a strong negative bias when rapidly transitioned to the cold high

DO hypolimnion. The sensor did not fully equilibrate after 7 minutes and ended at -0.8 mg/L

against the reference. For cast 2, there was a negative offset of -0.6 mg/L when equilibrated in the

hypolimnion and a positive bias when rapidly transitioned into the warm normoxic surface. The

sensor appeared to reach equilibration after 7 minutes but with a final offset of around 0.4 mg/L

against the reference. The range in measurement differences between instrument and reference

was -2.03 to 0.03 mg/L for cast 1 and -0.72 to 1.63 mg/L for cast 2.

BACKGROUND AND OBJECTIVES

Instrument performance verification is necessary so that effective existing technologies can

be recognized and so that promising new technologies can be made available to support coastal

science, resource management and ocean observing systems. To this end, the NOAA-funded

Alliance for Coastal Technologies (ACT) serves as an unbiased, third party testbed for evaluating

sensors and sensor platforms for use in coastal environments. ACT also serves as a comprehensive

data and information clearinghouse on coastal technologies and a forum for capacity building

through workshops on specific technology topics (visit

www.act-us.info

).

As part of our service to the coastal community, ACT conducted a performance verification

of commercially available, in situ dissolved oxygen (DO) sensors through the evaluation of

objective and quality assured data. The goal of ACT’s evaluation program is to provide technology

users with an independent and credible assessment of instrument performance in a variety of

environments and applications. To this end, the data and information on performance

characteristics were focused on the types of information users most need.

The fundamental objectives of this Performance Verification were to: (1) highlight the

potential capabilities of particular in situ DO sensors by demonstrating their utility in a range of

coastal environments; (2) verify the claims of manufacturers on the performance characteristics of

commercially available DO sensors when tested in a controlled laboratory setting, and (3) verify